Buster Moon (voiced by Matthew McConaughey), an optimistic koala, who plans to save his theater from closure by holding a singing competition.
Filled with an unrelenting drive to succeed, Buster Moon is determined to save his struggling theatre despite dwindling audiences, bad reviews and accumulating debt. Well-intentioned, he does have a habit of stretching the truth, which usually widens the hole he’s digging himself into. Nonetheless, he has an irresistible charm as his passion and optimism are utterly contagious.

Rosita (voiced by Reese Witherspoon), a domestic pig, who gave up her teenage music dreams to become a devoted wife of Norman, and mother to 25 piglets.
Rosita is a selfless homemaker and mother of 25 piglets who wants to remember what it feels like to be something other than a wife and a mom. In the competition, she’ll be partnered with a German pig named Gunter who instantly sees her inner diva and makes it his mission to unleash her.

Johnny (Taron Egerton) is a soulful, Cockney gorilla whose beautiful singing voice and passion for music are in direct opposition to his role in his father’s bank robbing crew. Deep down, Johnny wants to be a singer but knows that his alpha-male father would disown him if he knew the truth. He keeps his involvement in the competition secret, straddling a double life as an aspiring singer by day and getaway driver by night.

Ashley (Scarlett Johansson), a crested porcupine punk rocker who takes part in an alternative-rock music group with her boyfriend until she found out he was cheating on her.
is a prickly teenage porcupine with a punk rock attitude. She auditions for the competition with her egotistical, unsupportive jerk of a boyfriend, unable to see just how much he is holding her back. In rehearsals, her rebellious nature continually puts her at odds with Buster, who envisions her as a pop star princess whereas Ash wants to write her own authentic rock songs that reflect her unique style.

Gunter (Nick Kroll), a domestic pig and Rosita’s German-accented, very optimistic and bubbly dance partner.
Gunter is a boisterous dynamo and double threat singer-dancer who is partnered with Rosita to liven up her act. He feels most himself when rocking spandex dance leotards and encourages Rosita to loosen up and shed her inhibitions.

Mike (Seth MacFarlane) is a greedy, self-centered mouse with a Napoleon complex— and who happens to be a classically trained jazz musician with a voice like Sinatra. Driven by money, power and the allure of beautiful female mice, Mike is constantly trying to be part of a crowd that doesn’t want him… unless, that is, he can somehow win Buster’s competition

Meena (Tori Kelly), a teenage Indian elephant with an exquisite voice, and severe stage fright.
Meena is a vocal powerhouse hidden inside a shy teenage elephant with a devastating case of stage fright. After bungling her audition, she takes a job as the show’s stagehand, afraid to tell her family she didn’t make the cut. Ultimately, Buster will take Meena under his wing, hoping to help her overcome her anxiety, face her fears and deliver the show-stopping performance her family always believed she had within her.

Eddie Nana Noodleman (John C. Reilly), a Suffolk sheep, and Buster’s partner who doubts the future of the theater.

Norman (Nick Offerman), a domestic pig and Rosita’s workaholic husband.

Matilda Crawly (Garth Jennings), an elderly green iguana who is Buster’s assistant and offers Johnny piano lessons.

Marcus (Peter Serafinowicz), Johnny’s father who wants him to follow in his business of crime.

Lance (Beck Bennett), Ashley’s unfaithful boyfriend

Leslie Jones as Meena’s mother and Jay Pharoah as Meena’s Grandfather

Jewel, Martina McBride and Mýa as Marisa, Penny and Heather, the hares

John Robert Hall, Randy Newman and Scott Beaudin as Simon, Jerry and Benny, the frogs

– Jennifer Saunders as Miss Nana Noodleman, a Suffolk sheep who used to be a theater diva but who is now retired
– Jennifer Hudson as Young Nana Noodleman